Mouse D-Dimer,D2D ELISA Kit

Mouse D-Dimer,D2D ELISA Kit

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Mfr.No.

CSB-E13584m

Alternative Names

D-Dimer,D2D

Reactivity

Mouse

Detection Range

31.25 pg/mL-2000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	90-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-91
1:2	Average %	96
	Range %	91-102
1:4	Average %	84
	Range %	81-87
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
80	2.085	2.086	2.086	1.951
40	1.540	1.649	1.595	1.460
20	1.111	1.156	1.134	0.999
10	0.718	0.750	0.734	0.599
5	0.407	0.405	0.406	0.271
2.5	0.231	0.237	0.234	0.099
1.25	0.181	0.173	0.177	0.042
0	0.129	0.140	0.135	?

Species

Mus musculus

Sensitivity

7.81 pg/mL

Limit Of Detection

450 nm

Antigen	D-Dimer,D2D

Applications

ELISA

Research Area

D2D

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Thrombin-Activatable Fibrinolysis Inhibitor Protects Against Acute Lung Injury by Inhibiting the Complement System Naito M et al,Am J Respir Cell Mol Biol,2013 May 30. Comparison and evaluation of two different methods to establish the cigarette smoke exposure mouse model of COPD Shu J.et al,Sci Rep,Nov 13 Establishment and evaluation of COPD model by chronic exposure to MVE combined with LPS instillation Jiaze Shu.et al,experimental physiology,August 2

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SKU	Supplier No.	Size	Your price
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.