Mouse Cytotoxic T Lymphocyte Associated Antigen 4

Mfr.No.

E1996Mo

Sensitivity

0.058ng/mL

Detection Range

0.1-35ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CD antigen CD152;CTLA 4;CTLA4;CTLA-4;Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4

Molecular Weight

24993

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1996Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.8	0.26	5.3
Intra-Assay	2	18	11.3	0.51	4.5
Intra-Assay	3	18	3.3	0.13	4

Background

Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.

Protocol

These standard curves of E1996Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/mL	2.064	2.21	2.148
	2.356
10ng/mL	1.514	1.503	1.441
	1.492
5ng/mL	0.795	0.795	0.733
	0.795
2.5ng/mL	0.442	0.446	0.383
	0.449
1.25ng/mL	0.297	0.299	0.237
	0.302
0ng/mL	0.068	0.062	0
	0.056

Gene ID	12477
UniProt ID	P09793

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109808	E1996Mo	48T	$320		Add to Cart
BHE12109808	E1996Mo	96T	$458		Add to Cart

Mouse Cytotoxic T Lymphocyte Associated Antigen 4 - CTLA-4 ELISA Kit

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