Mouse Cytokeratin 18 - CK-18 ELISA Kit

Mfr.No.

E1671Mo

Sensitivity

0.023ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Mouse Cytokeratin 18 (also known as CK-18) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CK-18;Cytokeratin endo B;Cytokeratin-18;K18;Keratin D;Keratin, type I cytoskeletal 18;Keratin-18;KRT 18;KRT18;

Molecular Weight

47538

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1671Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.2	0.07	5.8
Intra-Assay	2	18	4.6	0.24	5.1
Intra-Assay	3	18	2.3	0.12	5.1

Background

When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Protocol

These standard curves of E1671Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.161	2.261	2.207
	2.361
6ng/ml	1.801	1.796	1.742
	1.791
3ng/ml	1.285	1.263	1.21
	1.242
1.5ng/ml	0.64	0.623	0.569
	0.605
0.75ng/ml	0.393	0.384	0.33
	0.375
0ng/ml	0.061	0.054	0
	0.046

Gene ID	16668
UniProt ID	P05784

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109512	E1671Mo	48T	$320		Add to Cart
BHE12109512	E1671Mo	96T	$458		Add to Cart

Mouse Cytokeratin 18 - CK-18 ELISA Kit

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