Mouse Cytochrome P450 2E1(CYP2E1) ELISA kit

Mouse Cytochrome P450 2E1(CYP2E1) ELISA kit

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Mfr.No.

CSB-EL006425MO

Alternative Names

cytochrome P450, family 2, subfamily E, polypeptide 1, CPE1, CYP2E, P450-J, P450C2E, cytochrome P450 2E1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, flavoprotein-linked monooxygenase, microsomal monooxygenase, xenobiotic monooxygenas

Reactivity

Mouse

Detection Range

39 pg/mL-2500 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat ANP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-95
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ANP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	97
	Range %	92-105
1:10	Average %	94
	Range %	89-97
1:20	Average %	91
	Range %	87-95
1:40	Average %	92
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.494	2.595	2.545	2.423
250	1.722	1.823	1.773	1.651
125	1.110	1.131	1.121	0.999
62.5	0.615	0.646	0.631	0.509
31.2	0.407	0.396	0.402	0.280
15.6	0.307	0.296	0.302	0.180
7.8	0.199	0.196	0.198	0.076
0	0.123	0.121	0.122	?

Species

Mus musculus

Sensitivity

9.75 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q05421
Antigen	cytochrome P450, family 2, subfamily E, polypeptide 1

Applications

ELISA

Research Area

CYP2E1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505546	CSB-EL006425MO	96T	$695	Add to Cart
BHE10505546	CSB-EL006425MO	5x96T	$3336	Add to Cart
BHE10505546	CSB-EL006425MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.