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Home / ELISA Kit

Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit

SKU : BHE13705196

The amount of PPARG, PPARG coactivator-1-alpha (PPARGC1A), ubiquinol-cytochrome c oxidoreductase core 2 subunit (UQCRC2), cytochrome c oxidase subunit I (MTCO1), uncoupling protein-2 (UCP2), and ATP synthase H(+)-transporting mitochondrial F1 complex (F1-ATP synthase) were markedly reduced in the low capacity runner rats in comparison with the high capacity runners.
The uniform decline in these proteins was consistent with the hypothesis that reduced aerobic metabolism plays a causal role in the development of the differences between the low capacity runner and high capacity runner rats. impairment of mitochondrial function may link reduced fitness to cardiovascular and metabolic disease.
Organism species: Homo sapiens (Human)

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Mfr.No.	KTE70034
Reactivity	Mouse
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCRC2.
Alternative Names	UQCRC2; QCR2; UQCR2;
Detection Method	Colorimetric
Kit Components	Mouse Cytochrome b-c1 complex subunit 2, mitochondrial microplate Mouse Cytochrome b-c1 complex subunit 2, mitochondrial standard Mouse Cytochrome b-c1 complex subunit 2, mitochondrial detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse UQCRC2. No significant cross-reactivity or interference between Mouse UQCRC2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The amount of PPARG, PPARG coactivator-1-alpha (PPARGC1A), ubiquinol-cytochrome c oxidoreductase core 2 subunit (UQCRC2), cytochrome c oxidase subunit I (MTCO1), uncoupling protein-2 (UCP2), and ATP synthase H(+)-transporting mitochondrial F1 complex (F1-ATP synthase) were markedly reduced in the low capacity runner rats in comparison with the high capacity runners. The uniform decline in these proteins was consistent with the hypothesis that reduced aerobic metabolism plays a causal role in the development of the differences between the low capacity runner and high capacity runner rats. impairment of mitochondrial function may link reduced fitness to cardiovascular and metabolic disease. Organism species: Homo sapiens (Human)
Notes	This Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCRC2 in samples. An antibody specific for UQCRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	67003

Applications	ELISA
Applications Notes	This Mouse Cytochrome b-c1 complex subunit 2, mitochondrial (UQCRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate UQCRC2 in samples. An antibody specific for UQCRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUQCRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UQCRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UQCRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13705196	KTE70034	48 T	$339		Add to Cart
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