Mouse Cystatin C,Cys-C ELISA Kit

Mouse Cystatin C,Cys-C ELISA Kit

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Mfr.No.

CSB-E08386m

Sensitivity

0.819 ng/mL

Detection Range

2.5 ng/mL-160 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

cystatin C, ARMD11, MGC117328, OTTHUMP00000164181, OTTHUMP00000164182, bA218C14.4 (cystatin C), cystatin 3, gamma-trace, neuroendocrine basic polypeptide, post-gamma-globulin

Recovery

The recovery of mouse LZM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-100
EDTA plasma (n=4)	91	85-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LZM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	93
	Range %	89-98
1:200	Average %	97
	Range %	94-101
1:400	Average %	98
	Range %	93-103
1:800	Average %	93
	Range %	87-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.523	2.456	2.490	2.300
25	1.845	1.869	1.857	1.667
12.5	1.267	1.365	1.316	1.126
6.25	0.802	0.829	0.816	0.626
3.12	0.483	0.486	0.485	0.295
1.56	0.345	0.362	0.354	0.164
0.78	0.266	0.269	0.268	0.078
0	0.192	0.188	0.190	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21460

Applications	ELISA
Research Area	CST3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10505537	CSB-E08386m	5x96T	$2376	Add to Cart
BHE10505537	CSB-E08386m	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.