Mouse CYP8B1 (7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase) ELISA Kit

Mfr.No.

EM2136

Alternative Names

CYP8B1 ELISA Kit, 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase ELISA Kit, 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase ELISA Kit, CYPVIIIB1 ELISA Kit, Cytochrome P450 8B1 ELISA Kit, Sterol 12-alpha-hydroxylase ELISA Kit

Reactivity

Mouse

Detection Method

Double Antibody

Detection Range

31.2-2000pg/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of CYP8B1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP8B1 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-105	96
EDTA plasma(n=5)	87-101	90
heparin plasma(n=5)	90-97	92

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP8B1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	91-105%	91-105%	94-105%
EDTA plasma(n=5)	89-97%	86-98%	86-98%
heparin plasma(n=5)	89-98%	88-100%	86-98%

Sensitivity

< 18.75pg/ml

Notes

For Research Use Only

UniProt ID	O88962

Applications	ELISA
Applications Notes	CYP8B1 ELISA Kit allows for the in vitro quantitative determination of CYP8B1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808921	EM2136	96T	$400		Add to Cart

Mouse CYP8B1 (7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase) ELISA Kit

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