Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of CYP7A1 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP7A1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
96
EDTA plasma(n=5)
85-103
95
heparin plasma(n=5)
85-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP7A1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CYP7A1 ELISA Kit allows for the in vitro quantitative determination of CYP7A1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse CYP7A1 (Cytochrome P450 7A1) ELISA Kit