Matrices listed below were spiked with certain level of Cyp2f2 and the recovery rates were calculated by comparing the measured value to the expected amount of Cyp2f2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-105
97
EDTA plasma(n=5)
89-103
97
heparin plasma(n=5)
88-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyp2f2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
86-104%
86-98%
EDTA plasma(n=5)
84-100%
84-98%
82-98%
heparin plasma(n=5)
82-97%
84-96%
81-89%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
P33267
Applications
ELISA
Applications Notes
Cyp2f2ELISA Kit allows for the in vitro quantitative determination of Cyp2f2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Cyp2f2 (Cytochrome P450 2F2) ELISA Kit