Matrices listed below were spiked with certain level of Cyp19a1 and the recovery rates were calculated by comparing the measured value to the expected amount of Cyp19a1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
95
EDTA plasma(n=5)
85-105
95
heparin plasma(n=5)
86-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cyp19a1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Cyp19a1 ELISA Kit allows for the in vitro quantitative determination of Cyp19a1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Cyp19a1(Cytochrome P450 19A1) ELISA Kit