Mouse cyclophilin A,CyPA ELISA Kit

Mouse cyclophilin A,CyPA ELISA Kit

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Mfr.No.

CSB-E09282m

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

peptidylprolyl isomerase A (cyclophilin A), CYPA, CYPH, MGC117158, MGC12404, MGC23397, PPIase A, T cell cyclophilin, cyclosporin A-binding protein, peptidyl-prolyl cis-trans isomerase A, peptidylprolyl isomerase A, rotamase A

Recovery

The recovery of rat SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	98-105
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	100
	Range %	95-105
1:10	Average %	96
	Range %	90-102
1:20	Average %	94
	Range %	88-99
1:40	Average %	90
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.762	2.803	2.783	2.589
500	2.117	2.104	2.111	1.917
250	1.598	1.583	1.591	1.397
125	1.103	1.136	1.120	0.926
62.5	0.652	0.672	0.662	0.468
31.25	0.416	0.426	0.421	0.227
15.6	0.341	0.351	0.346	0.152
0	0.193	0.195	0.194	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P17742

Applications	ELISA
Research Area	PPIA

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10505534	CSB-E09282m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.