Mouse Cubilin(CUBN) ELISA kit

Mouse Cubilin(CUBN) ELISA kit

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Mfr.No.

CSB-EL006213MO

Alternative Names

cubilin (intrinsic factor-cobalamin receptor), RP11-416D8.1, FLJ90055, FLJ90747, IFCR, MGA1, gp280, cubilin, intrinsic factor B12-receptor, intrinsic factor-cobalamin receptor, intrinsic factor-vitamin B12 receptor

Reactivity

Mouse

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	80-105
EDTA plasma (n=4)	94	82-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	81-102
1:2	Average %	96
	Range %	84-115
1:4	Average %	108
	Range %	90-110
1:8	Average %	95
	Range %	80-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml	OD1	OD2	Average	Corrected
70	2.835	2.697	2.766	2.612
35	1.930	1.955	1.943	1.789
17.5	1.146	1.159	1.153	0.999
8.75	0.673	0.701	0.687	0.533
4.4	0.457	0.446	0.452	0.298
2.2	0.321	0.326	0.324	0.170
1.1	0.243	0.246	0.245	0.091
0	0.156	0.152	0.154	?

Species

Mus musculus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q9JLB4
Antigen	cubilin (intrinsic factor-cobalamin receptor)

Applications

ELISA

Research Area

CUBN

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505523	CSB-EL006213MO	96T	$695	Add to Cart
BHE10505523	CSB-EL006213MO	5x96T	$3336	Add to Cart
BHE10505523	CSB-EL006213MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.