Mouse cross linked N-telopeptide of type I collagen,NTX ELISA Kit

Mfr.No.

CSB-E09242m

Alternative Names

cross linked N-telopeptide of type I collagen,NTX, cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX, cross linked N-telopeptide of type I collagen,NTX, cross linked N-telopeptide of type 1 collagen,NTX,_x000D_ cross linked N-telopeptide of type I collagen,NTX

Reactivity

Mouse

Detection Range

3.12 nM BCE-200 nM BCE

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rabbit Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-99
EDTA plasma (n=4)	95	89-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	81-97
1:2	Average %	89
	Range %	82-98
1:4	Average %	90
	Range %	85-100
1:8	Average %	95
	Range %	89-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pmol/ml	OD1	OD2	Average	?
1000	0.162	0.167	0.165	?
500	0.268	0.275	0.272	?
250	0.491	0.502	0.497	?
125	1.028	1.013	1.021	?
62.5	2.027	2.049	2.038	?

Species

Mus musculus

Sensitivity

0.78 nM BCE

Limit Of Detection

450 nm

Antigen	cross linked N-telopeptide of type Ⅰ collagen,NTX

Applications

ELISA

Research Area

NTX

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Pin1 Null Mice Exhibit Low Bone Mass and Attenuation of BMP Signaling Shen ZJ et al,PLoS One,2013 May 10;8(5):e63565.

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SKU	Supplier No.	Size	Your price
BHE10505514	CSB-E09242m	96T	$790	Add to Cart
BHE10505514	CSB-E09242m	5x96T	$3792	Add to Cart
BHE10505514	CSB-E09242m	10x96T	$7505	Add to Cart

Mouse cross linked N-telopeptide of type I collagen,NTX ELISA Kit

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