Mouse cross linked C-telopeptide of type I collagen (CTX-I) ELISA Kit

Mfr.No.

CSB-E12782m

Sensitivity

0.04 ng/mL

Detection Range

0.075 ng/mL-15 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

cross linked C-telopeptide of type I collagen (CTX-I), cross linked C-telopeptide of type 1 collagen (CTX-1),_x000D_ cross linked C-telopeptide of type I collagen (CTX-I), cross linked C-telopeptide of type I collagen (CTX-I), cross linked C-telopeptide of type 1 collagen (CTX-1),_x000D_ cross linked C-telopeptide of type I collagen (CTX-I)

Recovery

The recovery of human ERRFI1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-102
EDTA plasma (n=4)	89	86-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ERRFI1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	102
	Range %	96-107
1:4	Average %	97
	Range %	94-102
1:8	Average %	87
	Range %	83-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	1.853	1.916	1.885	1.771
1000	1.387	1.452	1.420	1.306
500	0.875	0.918	0.897	0.783
250	0.552	0.577	0.565	0.451
125	0.352	0.361	0.357	0.243
62.5	0.246	0.253	0.250	0.136
31.25	0.167	0.173	0.170	0.056
0	0.113	0.115	0.114	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	CTX-I

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Altered bone development in a mouse model of peripheral sensory nerve inactivation. Heffner MA et al,J Musculoskelet Neuronal Interact,2014 Mar;14(1):1-9. HYPOXIA-REPERFUSION AFFECTS OSTEOGENIC LINEAGE AND PROMOTES SICKLE CELL BONE DISEASE Dalle Carbonare L. et al,Blood,2015 126(20):2320-8. Effect of alendronate on post-traumatic osteoarthritis induced by anterior cruciate ligament rupture in mice Khorasani MS.et al,Arthritis Res Ther.,2015 17:30. Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-a-induced osteoclastogenesis and promoting osteoblastic differentiation in mice Noguchi T. et al,Biochem Biophys Res Commun,2015 Aug 20.pii: S0006-291X(15)30467-8. Progranulin plays crucial roles in preserving bone mass by inhibiting TNF-a-induced osteoclastogenesis and promoting osteoblastic differentiation in mice Noguchi T. et al,Biochem Biophys Res Commun,2015 465(3):638-43. A Novel Diterpenoid Suppresses Osteoclastogenesis and Promotes Osteogenesis by Inhibiting Ifrd1-and IkBa-Mediated p65 Nuclear Translocation /,Journal of Bone and Mineral Research ,Nov 1 VEGF-C promotes the development of lymphatics in bone and bone loss Devon Hominick.et al,Elife. 2018,5 April Apolipoprotein E plays crucial roles in maintaining bone mass by promoting osteoblast differentiation via ERK1/2 pathway and by suppressing osteoclast differentiation via c-Fos, NFATc1, and NF-κB pathway TakaakiNoguchi.et al,Biochemical and Biophysical Research Communications,June 15 A novel anti-osteoporotic agent that protects against postmenopausal bone loss by regulating bone formation and bone resorption Yu-te Yang .et al,Life Sciences,August 7

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Mouse cross linked C-telopeptide of type I collagen (CTX-I) ELISA Kit

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