Matrices listed below were spiked with certain level of Crh and the recovery rates were calculated by comparing the measured value to the expected amount of Crh in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-99
92
EDTA plasma(n=5)
86-102
93
heparin plasma(n=5)
89-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Crh and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Crh ELISA Kit allows for the in vitro quantitative determination of Crh concentrations in serum, plasma, tissue homogenates and other biological fluids.