Mouse Creatine Kinase B-type

Mfr.No.

E1978Mo

Sensitivity

0.232mU/mL

Detection Range

0.5-150mU/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

B-CK;CKB;CPK-B;Creatine kinase B chain;Creatine kinase B-type;Creatine phosphokinase B-type

Molecular Weight

42713

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1978Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.8	1.04	5.9
Intra-Assay	2	18	56.1	2.19	3.9
Intra-Assay	3	18	21.6	0.98	4.5

Background

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Protocol

These standard curves of E1978Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80mU/mL	2.353	2.246	2.215
	2.138
40mU/mL	1.294	1.37	1.339
	1.445
20mU/mL	0.699	0.627	0.597
	0.555
10mU/mL	0.34	0.372	0.342
	0.405
5mU/mL	0.193	0.196	0.165
	0.198
0mU/mL	0.029	0.031	0
	0.032

Gene ID	12709
UniProt ID	Q04447

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109790	E1978Mo	48T	$320		Add to Cart
BHE12109790	E1978Mo	96T	$458		Add to Cart

Mouse Creatine Kinase B-type - CKB ELISA Kit

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