Mouse Cramp ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15061m

Sensitivity

0.39 pg/mL

Detection Range

1.56 pg/mL-100 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Cathelicidin antimicrobial peptide, CAP18, CRAMP, FALL-39, FALL39, HSD26, LL37, CAMP

Recovery

The recovery of human AFU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	95-110
EDTA plasma (n=4)	93	85-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AFU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	88
	Range %	81-95
1:40	Average %	91
	Range %	86-97
1:80	Average %	97
	Range %	91-102
1:160	Average %	102
	Range %	97-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P51437

Applications	ELISA
Research Area	CAMP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Double-stranded RNA induces cathelicidin expression in the intestinal epithelial cells through phosphatidylinositol 3-kinase-protein kinase Cζ-Sp1 pathway and ameliorates shigellosis in mice Atri Ta.et al,Cell Signal. ,Mar 24;35:140-153. Allergen specific immunotherapy enhanced defense against bacteria via TGF-B1-induced CYP27B1 in asthma Wang J.et al,Oncotarget.,Aug 2

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