Mouse Corticotropin-releasing Factor(CRF) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14068m

Alternative Names

corticotropin releasing hormone, CRF, corticotropin-releasing factor

Reactivity

Mouse

Detection Range

2.5 pg/mL-40 pg/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human pepsinogen spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	86	81-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human pepsinogen in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	102
	Range %	96-107
1:2	Average %	94
	Range %	90-98
1:4	Average %	95
	Range %	89-99
1:8	Average %	93
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.675 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8CIT0
Antigen	Corticotropin Releasing Hormone

Applications

ELISA

Research Area

CRH

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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