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Home / ELISA Kit

Mouse Corticotropin-releasing Factor-binding Protein - CRHBP ELISA Kit

SKU : BHE12109784

Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E1972Mo

Sensitivity

5.19ng/L

Detection Range

10-2000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Corticotropin-releasing factor-binding protein;Corticotropin-releasing hormone-binding protein;CRF-binding protein;CRF-BP;CRHBP;CRH-BP

Molecular Weight

36048

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1972Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	96.7	4.32	4.5
Intra-Assay	2	18	205	8.08	3.9
Intra-Assay	3	18	391.2	22.06	5.6

Background

Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.

Protocol

These standard curves of E1972Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.112	2.144	2.111
	2.176
600ng/L	1.785	1.783	1.75
	1.781
300ng/L	1.182	1.201	1.168
	1.22
150ng/L	0.699	0.669	0.636
	0.638
75ng/L	0.43	0.426	0.393
	0.421
0ng/L	0.028	0.033	0
	0.038

Gene ID	12919
UniProt ID	Q60571

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109784	E1972Mo	48T	$320		Add to Cart
BHE12109784	E1972Mo	96T	$458		Add to Cart

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