Mouse Corticotropin-releasing factor-binding protein(CRHBP) ELISA kit

Mfr.No.

CSB-EL005964MO

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

corticotropin releasing hormone binding protein, CRF-BP, CRFBP, corticotropin releasing hormone-binding protein

Recovery

The recovery of human TREML1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	84-97
EDTA plasma (n=4)	96	87-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TREML1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-94
1:2	Average %	100
	Range %	95-104
1:4	Average %	97
	Range %	86-102
1:8	Average %	102
	Range %	94-107

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q60571

Applications	ELISA
Research Area	CRHBP

Storage	Store at 2-8°C. Please refer to protocol.

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