Mouse Complement fragment 3a,C3a ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08511m

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Complement fragment 3a,C3a

Recovery

The recovery of human OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-104
EDTA plasma (n=4)	94	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	99
	Range %	93-105
1:200	Average %	94
	Range %	91-98
1:400	Average %	103
	Range %	99-107
1:800	Average %	86
	Range %	80-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	C3a

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10505489	CSB-E08511m	96T	$695	Add to Cart
BHE10505489	CSB-E08511m	5x96T	$3336	Add to Cart
BHE10505489	CSB-E08511m	10x96T	$6602.50	Add to Cart