Mouse Complement Factor H/CFH ELISA Kit PicoKine®

Mfr.No.

EK1400

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.001	0.215	0.332	0.578	0.971	1.289	1.609	1.938

Description

Mouse Complement Factor H/CFH ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cfh in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement factor H;Protein beta-1-H;Cfh;Hf1;

Molecular Weight

139138 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S875-V1252

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CFH

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1657	6025	19536	1601	5467	19303
Standard deviation	120.96	343.42	1133.08	124.87	371.75	1351.21
CV (%)	7.3%	5.7%	5.8%	7.8%	6.8%	7%

Protein Function

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alteative complement pathway. .

Background

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alteativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alteative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1657	1662	1609	1498	1606	65.97	4.1%
Sample 2	6025	6672	6459	5668	6206	388.38	6.2%
Sample 3	19536	22857	19293	22922	21152	1739.77	8.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12628
Gene Name	Cfh
UniProt ID	P06909
Protein Name	Complement factor H
Tissue Specificity	Expressed by the liver and secreted in plasma.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000997	EK1400	96 wells/kit	$499		Add to Cart

Mouse Complement Factor H/CFH ELISA Kit PicoKine®

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