Mouse Complement Factor D

Mfr.No.

E1969Mo

Sensitivity

0.29ng/mL

Detection Range

0.5-200ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

28 kDa adipocyte protein;Adipsin;C3 convertase activator;CFD;Complement factor D;Properdin factor D

Molecular Weight

28057

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1969Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	69.5	3.17	4.6
Intra-Assay	2	18	42.5	1.84	4.3
Intra-Assay	3	18	23.1	1.15	5

Background

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.

Protocol

These standard curves of E1969Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/mL	2.27	2.118	2.077
	1.967
60ng/mL	1.393	1.482	1.44
	1.571
30ng/mL	0.692	0.707	0.665
	0.721
15ng/mL	0.378	0.343	0.301
	0.308
7.5ng/mL	0.158	0.148	0.107
	0.139
0ng/mL	0.028	0.042	0
	0.055

Gene ID	11537
UniProt ID	P03953

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109781	E1969Mo	48T	$320		Add to Cart
BHE12109781	E1969Mo	96T	$458		Add to Cart

Mouse Complement Factor D - CFD ELISA Kit

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