Mouse Complement C2(C2) ELISA kit

Mfr.No.

CSB-EL003658MO

Sensitivity

18.75 ng/mL

Detection Range

18.75 ng/mL-300 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

complement component 2, DADB-122G4.1, CO2, DKFZp779M0311, C3/C5 convertase, complement component C2

Recovery

The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	84-95
EDTA plasma (n=4)	108	97-112

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	107
	Range %	95-111
1:2	Average %	100
	Range %	93-104
1:4	Average %	94
	Range %	86-97
1:8	Average %	100
	Range %	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P21180

Applications	ELISA
Research Area	C2

Storage	Store at 2-8°C. Please refer to protocol.

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