Mouse Complement C1q subcomponent subunit A(C1QA) ELISA kit

Mouse Complement C1q subcomponent subunit A(C1QA) ELISA kit

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Mfr.No.

CSB-EL003637MO

Sensitivity

0.039 ug/mL

Detection Range

0.156 ug/mL-10 ug/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement component 1, q subcomponent, A chain, OTTHUMP00000197085, complement component 1, q subcomponent, alpha polypeptide, complement component C1q, A chain, complement component 1, q subcomponent, A chain, OTTHUMP00000197085, complement component 1, q subcomponent, alpha polypeptide, complement component C1q, A chain

Recovery

The recovery of human PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	86-97
EDTA plasma (n=4)	96	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	95
	Range %	92-101
1:40	Average %	93
	Range %	83-99
1:80	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
400	2.548	2.452	2.500	2.294
200	2.196	2.076	2.136	1.930
100	1.632	1.593	1.613	1.407
50	1.082	1.122	1.102	0.896
25	0.675	0.645	0.660	0.454
12.5	0.441	0.436	0.439	0.233
6.25	0.320	0.315	0.318	0.112
0	0.205	0.207	0.206	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P98086

Applications	ELISA
Research Area	C1QA

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505467	CSB-EL003637MO	96T	$695	Add to Cart
BHE10505467	CSB-EL003637MO	5x96T	$3336	Add to Cart
BHE10505467	CSB-EL003637MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.