Mouse Complement 4,C4 ELISA Kit

Mouse Complement 4,C4 ELISA Kit

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Mfr.No.

CSB-E08707m

Sensitivity

0.039 ug/mL

Detection Range

0.156 ug/mL-10 ug/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

complement component 4B (Chido blood group), DADB-112B14.11, C4B1, C4B12, C4B2, C4B3, C4B5, C4F, CH, CO4, CPAMD3, FLJ60561, MGC164979, Chido form of C4, basic C4, complement C4B1a, complement component 4B

Recovery

The recovery of rabbit OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	97	90-108

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	101
	Range %	91-105
1:4	Average %	99
	Range %	92-110
1:8	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?mol/ml	OD1	OD2	Average	Corrected
5	2.646	2.606	2.626	2.548
2.5	2.127	2.113	2.120	2.042
1.25	1.453	1.345	1.399	1.321
0.625	0.832	0.827	0.830	0.752
0.312	0.431	0.424	0.428	0.350
0.156	0.234	0.222	0.228	0.150
0.078	0.143	0.133	0.138	0.060
0	0.080	0.076	0.078	?

Species

Mus musculus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01029

Applications	ELISA
Research Area	C4B

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505462	CSB-E08707m	96T	$695	Add to Cart
BHE10505462	CSB-E08707m	5x96T	$3336	Add to Cart
BHE10505462	CSB-E08707m	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.