Mouse Collagen Type IV,Col IV ELISA KIT

Mouse Collagen Type IV,Col IV ELISA KIT

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Mfr.No.

CSB-E08884m

Alternative Names

Collagen Type IV,Col IV, Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV, Collagen Type IV,Col IV, Collagen Type 4,Col 4,_x000D_ Collagen Type IV,Col IV

Reactivity

Mouse

Detection Range

0.78 ng/mL-50 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TRAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	86-95
EDTA plasma (n=4)	99	95-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TRAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	96
	Range %	92-103
1:4	Average %	102
	Range %	95-108
1:8	Average %	88
	Range %	86-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.254	2.425	2.340	2.203
20	1.834	1.896	1.865	1.728
10	1.362	1.422	1.392	1.255
5	0.965	1.012	0.989	0.852
2.5	0.633	0.629	0.631	0.494
1.25	0.382	0.354	0.368	0.231
0.625	0.266	0.245	0.256	0.119
0	0.133	0.140	0.137

Species

Mus musculus

Sensitivity

0.195 ng/mL

Limit Of Detection

450 nm

Antigen	Collagen Type Ⅳ,Col Ⅳ

Applications

ELISA

Research Area

Col IV

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Tongxinluo Inhibits Renal Fibrosis in Diabetic Nephropathy: Involvement of the Suppression of Intercellular Transfer of TGF-[Formula: see text]1-Containing Exosomes from GECs to GMCs. Wu XM.et al,Am J Chin Med,Jun 28

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BHE10505455	CSB-E08884m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.