Mouse Collagen Type III,Col III ELISA KIT

Mfr.No.

CSB-E07925m

Alternative Names

Collagen Type III,Col III, Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III, Collagen Type III,Col III, Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III

Reactivity

Mouse

Detection Range

3.12 pg/mL-200 pg/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CD147 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	107	103-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD147 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-104
1:2	Average %	94
	Range %	89-98
1:4	Average %	96
	Range %	93-100
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.112	2.202	2.157	2.053
400	1.756	1.735	1.746	1.642
200	1.142	1.201	1.172	1.068
100	0.725	0.741	0.733	0.629
50	0.413	0.405	0.409	0.305
25	0.278	0.281	0.280	0.176
12.5	0.211	0.200	0.206	0.102
0	0.105	0.103	0.104	?

Species

Mus musculus

Sensitivity

0.78 pg/mL

Limit Of Detection

450 nm

Antigen	Collagen Type Ⅲ,Col Ⅲ

Applications

ELISA

Research Area

Col III

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Development of a Novel Severe Triple Allergen Asthma Model in Mice Which Is Resistant to Dexamethasone and Partially Resistant to TLR7 and TLR9 Duechs MJ et al,PLoS One,2014 Mar 11;9(3):e91223.

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SKU	Supplier No.	Size	Your price
BHE10505454	CSB-E07925m	96T	$835	Add to Cart
BHE10505454	CSB-E07925m	5x96T	$4008	Add to Cart
BHE10505454	CSB-E07925m	10x96T	$7932.50	Add to Cart

Mouse Collagen Type III,Col III ELISA KIT

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