Mouse Collagen Type II,Col II ELISA Kit

Mfr.No.

CSB-E08089m

Sensitivity

0.46 ng/mL

Detection Range

1.87 ng/mL-120 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type II,Col II, Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II, Collagen Type II,Col II, Collagen Type 2,Col 2,_x000D_ Collagen Type II,Col II

Recovery

The recovery of human BRDT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BRDT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	87
	Range %	82-91
1:4	Average %	105
	Range %	101-113
1:8	Average %	97
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.751	2.593	2.672	2.541
2000	1.875	1.758	1.817	1.686
1000	1.040	1.070	1.055	0.924
500	0.592	0.611	0.602	0.471
250	0.364	0.349	0.357	0.226
125	0.239	0.227	0.233	0.102
62.5	0.158	0.165	0.162	0.031
0	0.127	0.134	0.131	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Col II

Storage	Store at 2-8°C. Please refer to protocol.

Reference	COMPOSITION AND KIT FOR PREVENTION OR TREATMENT OF ARTHRITIS, AND METHOD USING THE SAME Tanner GJ.et al,PLoS One,08/10/2017

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SKU	Supplier No.	Size	Your price
BHE10505453	CSB-E08089m	96T	$835	Add to Cart
BHE10505453	CSB-E08089m	5x96T	$4008	Add to Cart
BHE10505453	CSB-E08089m	10x96T	$7932.50	Add to Cart

Mouse Collagen Type II,Col II ELISA Kit

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