Mouse collagen type II(Col II) antibody ELISA kit

Mouse collagen type II(Col II) antibody ELISA kit

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Mfr.No.

CSB-EQ027975MO

Sensitivity

0.312 U/mL

Detection Range

0.312 U/mL-20 U/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

collagen type II(Col II) antibody, collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody, collagen type II(Col II) antibody, collagen type 2(Col 2) antibody,_x000D_ collagen type II(Col II) antibody

Recovery

The recovery of pig FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	97	91-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-94
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	87-97
1:8	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
700	1.389	1.416	1.403	1.367
250	0.578	0.615	0.597	0.561
100	0.317	0.302	0.310	0.274
50	0.163	0.154	0.159	0.123
20	0.095	0.101	0.098	0.062
0	0.037	0.034	0.036	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col II Ab

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505452	CSB-EQ027975MO	96T	$695	Add to Cart
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BHE10505452	CSB-EQ027975MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.