Mouse Collagen Type I,Col IELISA Kit

Mouse Collagen Type I,Col IELISA Kit

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Mfr.No.

CSB-E08083m

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Collagen Type I,Col I, Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I, Collagen Type I,Col I, Collagen Type 1,Col 1,_x000D_ Collagen Type I,Col I

Recovery

The recovery of human BRD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-107
EDTA plasma (n=4)	85	80-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BRD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	87
	Range %	80-93
1:4	Average %	90
	Range %	83-98
1:8	Average %	95
	Range %	90-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	1.963	1.903	1.933	1.779
600	1.624	1.573	1.599	1.445
300	1.217	1.182	1.200	1.046
150	0.952	0.934	0.943	0.789
75	0.571	0.526	0.549	0.395
37.5	0.335	0.317	0.326	0.172
18.75	0.206	0.199	0.203	0.049
0	0.159	0.148	0.154	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	Col I

Storage	Store at 2-8°C. Please refer to protocol.

Reference	A1M Ameliorates Preeclampsia-Like Symptoms in Placenta and Kidney Induced by Cell-Free Fetal Hemoglobin in Rabbit. Naumlv.et al,PLoS One,2015 May 8;10(5):e0125499. Carboxymethyl chitosan nanoparticles loaded with bioactive peptide OH-CATH30 benefit nonscar wound healing Sun T.et al,International Journal of Nanomedicine,September 25

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SKU	Supplier No.	Size	Your price
BHE10505451	CSB-E08083m	96T	$835	Add to Cart
BHE10505451	CSB-E08083m	5x96T	$4008	Add to Cart
BHE10505451	CSB-E08083m	10x96T	$7932.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.