Mouse Collagen alpha-1(V) chain (COL5A1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL005748MO

Alternative Names

collagen, type V, alpha 1, Col5a1Collagen alpha-1(V) chain ELISA kit, collagen, type V, alpha 1, Col5a1Collagen alpha-1(V) chain ELISA kit

Reactivity

Mouse

Detection Range

0.312 ng/ml -20 ng/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	93	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	90
	Range %	86-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	97
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Mus musculus

Sensitivity

0.078 ng/ml

Limit Of Detection

450 nm

UniProt ID	O88207
Antigen	collagen, type V, alpha 1

Applications

ELISA

Research Area

COL5A1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10505427	CSB-EL005748MO	96T	$695	Add to Cart
BHE10505427	CSB-EL005748MO	5x96T	$3336	Add to Cart
BHE10505427	CSB-EL005748MO	10x96T	$6602.50	Add to Cart