Mouse Cold-inducible RNA-binding Protein

Mfr.No.

E1951Mo

Sensitivity

2.24ng/L

Detection Range

5-1000ng/L

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

A18 hnRNP;CIRBP;Cirp;Cold-inducible RNA-binding protein;Glycine-rich RNA-binding protein CIRP;R74941

Molecular Weight

18607

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1951Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	370.85	14.22	3.83
Intra-Assay	2	18	268.21	12.74	4.75
Intra-Assay	3	18	157.12	7.67	4.88

Background

Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Promotes assembly of stress granules (SGs), when overexpressed. Seems to play an essential role in cold-induced suppression of cell proliferation. Acts as a translational repressor. Acts as a translational activator. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN.

Protocol

These standard curves of E1951Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	1.952	2.093	2.054
	2.234
320ng/L	1.45	1.385	1.347
	1.32
160ng/L	0.587	0.654	0.616
	0.72
80ng/L	0.399	0.38	0.342
	0.361
40ng/L	0.164	0.144	0.106
	0.124
0ng/L	0.021	0.038	0
	0.056

Gene ID	12696
UniProt ID	P60824

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109763	E1951Mo	48T	$320		Add to Cart
BHE12109763	E1951Mo	96T	$458		Add to Cart

Mouse Cold-inducible RNA-binding Protein - CIRBP ELISA Kit

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