Mouse COL1a1(Collagen Type I Alpha 1) ELISA Kit

Mfr.No.

ELK3453

Alternative Names

COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain

Reactivity

Mouse

Detection Range

0.16-10 ng/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant COL1a1 and the recovery rates were calculated by comparing the measured value to the expected amount of COL1a1 in samples.

Matrix	Recovery range	Average
serum(n=5)	88-103%	95%
EDTA plasma(n=5)	92-106%	99%
Heparin plasma(n=5)	90-105%	97%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of COL1a1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-92%	89-103%	97-105%	91-99%
EDTA plasma(n=5)	88-102%	86-92%	81-93%	89-104%
Heparin plasma(n=5)	95-104%	79-92%	82-95%	86-97%

Typical Data

<table> <tbody><tr> <th>Concentration (ng/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>10.00</td> <td>1.948</td> <td>1.871</td> </tr> <tr> <td>5.00</td> <td>1.630</td> <td>1.553</td> </tr> <tr> <td>2.50</td> <td>1.174</td> <td>1.097</td> </tr> <tr> <td>1.25</td> <td>0.757</td> <td>0.680</td> </tr> <tr> <td>0.63</td> <td>0.521</td> <td>0.444</td> </tr> <tr> <td>0.32</td> <td>0.372</td> <td>0.295</td> </tr> <tr> <td>0.16</td> <td>0.199</td> <td>0.122</td> </tr> <tr> <td>0.00</td> <td>0.077</td> <td>0.000</td> </tr> </tbody></table>

Sensitivity

0.067 ng/mL

Limit Of Detection

10 ng/mL

UniProt ID	P11087
Antigen	COL1a1
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL1a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL1a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL1a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL1a1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Metabolic pathway;
Protocol	<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3453-1.pdf" target="_blank">Datasheet</a>
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15206739	ELK3453	48T	$320		Add to Cart
BHE15206739	ELK3453	96T	$458		Add to Cart

Mouse COL1a1(Collagen Type I Alpha 1) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Mouse COL1a1(Collagen Type I Alpha 1) ELISA Kit

Rate it now..!!