Mouse coagulation factor VII,FVII ELISA Kit

Mouse coagulation factor VII,FVII ELISA Kit

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Mfr.No.

CSB-E17817m

Sensitivity

0.11 ng/mL

Detection Range

0.45 ng/mL-30 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

coagulation factor VII (serum prothrombin conversion accelerator), FVII coagulation protein, coagulation factor VII, eptacog alfa, coagulation factor 7,F7, coagulation factor VII (serum prothrombin conversion accelerator), FVII coagulation protein, coagulation factor VII, eptacog alfa, coagulation factor 7,F7

Recovery

The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	86	82-91

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-99
1:2	Average %	94
	Range %	91-98
1:4	Average %	101
	Range %	97-105
1:8	Average %	86
	Range %	82-90

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	1.851	1.992	1.922	1.878
10	1.058	1.136	1.097	1.053
4.375	0.502	0.524	0.513	0.469
1.875	0.268	0.255	0.262	0.218
0.625	0.116	0.119	0.118	0.074
0	0.043	0.045	0.044	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P70375

Applications	ELISA
Research Area	F7

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.