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Home / ELISA Kit

Mouse Coagulation Factor 8 - F8 ELISA Kit

SKU : BHE12109758

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

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Bioassay Technology Laboratory

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Tel

+1 866.986.9598

Email

[email protected]

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Mfr.No.

E1946Mo

Sensitivity

0.022ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Mouse

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Coagulation factor VIII;F8;Procoagulant component

Molecular Weight

266196

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1946Mo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.7	0.22	4.8
Intra-Assay	2	18	9.8	0.25	2.6
Intra-Assay	3	18	2.4	0.11	4.8

Background

Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

Protocol

These standard curves of E1946Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	1.931	1.912	1.868
	1.893
6ng/mL	0.872	0.93	0.886
	0.989
3ng/mL	0.554	0.585	0.541
	0.615
1.5ng/mL	0.231	0.203	0.159
	0.175
0.75ng/mL	0.105	0.103	0.059
	0.101
0ng/mL	0.05	0.044	0
	0.038

Gene ID	14069
UniProt ID	Q06194

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12109758	E1946Mo	48T	$320		Add to Cart
BHE12109758	E1946Mo	96T	$458		Add to Cart

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