Mouse CNP (C-type natriuretic peptide) ELISA Kit

Mfr.No.

E-EL-M0370

Sensitivity

9.38 pg/mL

Detection Range

15.63-1000 pg/mL

Reactivity

Mouse

Assay Duration

2.5h

Assay Type

Competitive

Description

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with CNP. During the reaction, CNP in the sample or standard competes with a fixed amount of CNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to CNP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CNP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Detection Method

Colormetric

Kit Components

An unopened kit can be stored at 2-8°C for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells x12 strips 48T: 8 wells x6 strips	-20°C, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL
Concentrated HRP Conjugate (100x)	96T: 1 vial, 120 uL 48T: 1 vial, 60 uL	-20°C(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25x)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8°C(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Recovery

80%-120%. The recovery of CNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-99	94
EDTA plasma(n=8)	94-107	99
Cell culture media(n=8)	93-106	100

Linearity

Samples were spiked with high concentrations of Mouse CNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	99-114	91-104	98-115
1:2	Average (%)	106	98	105
1:4	Range (%)	85-98	93-105	106
1:4	Average (%)	92	99	106
1:8	Range (%)	85-99	91-105	96-111
1:8	Average (%)	90	97	102
1:16	Range (%)	85-100	91-106	93-105
1:16	Average (%)	91	99	100

Specificity

This kit recognizes CNP in samples. No significant cross-reactivity or interference between CNP and analogues was observed.

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	4℃(shading light)
Stop Solution	1 vial, 10 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Precision

Both intra-CV and inter-CV are < 10%.
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level CNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level CNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	51.20	150.10	345.40	54.10	162.30	320.10
Standard deviation	2.90	8.00	16.90	3.80	9.10	14.40
CV (%)	5.66	5.33	4.89	7.02	5.61	4.50

Background

This kit uses competitive-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with Mouse CNP .During the reaction, Mouse CNP in the sample or standard competes with a fixed amount of Mouse CNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse CNP Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse CNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Mouse CNP concentrations in Serum, plasma and other biological fluids.

UniProt ID	Q61839

Applications	ELISA
Applications Notes	This ELISA kit applies to the in vitro quantitative determination of CNP concentrations in Serum, plasma and other biological fluids.
Research Area	Cancer, Neuroscience, Tags and Cell Markers

Storage	2-8°C/-20°C,12 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10302643	E-EL-M0370	96T	$609		Add to Cart
BHE10302643	E-EL-M0370	48T	$487		Add to Cart

Mouse CNP (C-type natriuretic peptide) ELISA Kit

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