Mouse Clusterin(CLU) ELISA kit

Mouse Clusterin(CLU) ELISA kit

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Mfr.No.

CSB-EL005595MO

Alternative Names

clusterin, AAG4, APOJ, CLI, KUB1, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, aging-associated protein 4, apolipoprotein J, clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone

Reactivity

Mouse

Detection Range

1.563 ng/mL-100 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse MMP7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	92-96
EDTA plasma (n=4)	96	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MMP7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-95
1:2	Average %	102
	Range %	98-104
1:4	Average %	92
	Range %	88-96
1:8	Average %	99
	Range %	95-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.180	2.088	2.134	1.981
1000	1.548	1.534	1.541	1.388
500	0.882	0.899	0.891	0.738
250	0.511	0.489	0.500	0.347
125	0.338	0.331	0.335	0.182
62.5	0.256	0.248	0.252	0.099
31.25	0.202	0.211	0.207	0.054
0	0.154	0.152	0.153	?

Species

Mus musculus

Sensitivity

0.277 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q06890
Antigen	Clusterin

Applications

ELISA

Research Area

CLU

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505396	CSB-EL005595MO	96T	$495	Add to Cart
BHE10505396	CSB-EL005595MO	5x96T	$2376	Add to Cart
BHE10505396	CSB-EL005595MO	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.