Mouse Cluster of differentiation 30,CD30 ELISA Kit

Mouse Cluster of differentiation 30,CD30 ELISA Kit

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Mfr.No.

CSB-E04525m

Sensitivity

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Detection Range

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Reactivity

Mouse

Assay Duration

1-5h

Sample Volume

50-100ul

Alternative Names

tumor necrosis factor receptor superfamily, member 8, CD30, D1S166E, Ki-1, CD30 antigen, CD30L receptor, Ki-1 antigen, cytokine receptor CD30, lymphocyte activation antigen CD30

Recovery

The recovery of mouse WNT5A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-98
EDTA plasma (n=4)	98	95-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse WNT5A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-98
1:2	Average %	98
	Range %	92-106
1:4	Average %	94
	Range %	88-98
1:8	Average %	88
	Range %	83-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.755	2.652	2.704	2.536
100	2.324	2.376	2.350	2.182
50	1.639	1.752	1.696	1.528
25	0.954	1.035	0.995	0.827
12.5	0.565	0.524	0.545	0.377
6.25	0.372	0.359	0.366	0.198
3.12	0.257	0.259	0.258	0.090
0	0.171	0.165	0.168	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

UniProt ID	Q60846
Tissue Specificity	Detected in thymus and in activated splenocytes.

Applications	ELISA
Research Area	TNFRSF8

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505398	CSB-E04525m	96T	$595	Add to Cart
BHE10505398	CSB-E04525m	5x96T	$2856	Add to Cart
BHE10505398	CSB-E04525m	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.