Matrices listed below were spiked with certain level of CLDN5 and the recovery rates were calculated by comparing the measured value to the expected amount of CLDN5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
95
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
85-104
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CLDN5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-104%
87-103%
85-105%
EDTA plasma(n=5)
88-97%
89-101%
84-99%
heparin plasma(n=5)
86-98%
80-94%
81-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O54942
Applications
ELISA
Applications Notes
CLDN5 ELISA Kit allows for the in vitro quantitative determination of CLDN5 concentrations in serum, plasma, tissue homogenates and other biological fluids.