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Home / ELISA Kit

Mouse Clara cell protein,CC16 ELISA Kit

SKU : BHE10505394

This Human BNP ELISA Kit was designed for the quantitative measurement of Human BNP protein in serum, urine, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 93.75 pg/mL-6000 pg/mL and the sensitivity is 23.43 pg/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-E08682m

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Mouse

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Clara cell protein,CC16, CC10, CC16, CCSP, UGB, Uteroglobin (Clara-cell specific 10-kD protein) SCGB1A1)

Recovery

The recovery of human BNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		96		90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human BNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		86
		Range %		81-92
1:2		Average %		95
		Range %		90-105
1:4		Average %		95
		Range %		91-110
1:8		Average %		93
		Range %		86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.311	2.256	2.284	2.179
3000	1.905	1.876	1.891	1.786
1500	1.548	1.447	1.498	1.393
750	1.102	1.059	1.081	0.976
375	0.871	0.857	0.864	0.759
187.5	0.502	0.490	0.496	0.391
93.75	0.313	0.297	0.305	0.200
0	0.109	0.101	0.105	?

Species

Mus musculus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	Q06318

Applications	ELISA
Research Area	CC16

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10505394	CSB-E08682m	96T	$835		Add to Cart
BHE10505394	CSB-E08682m	5x96T	$4008		Add to Cart
BHE10505394	CSB-E08682m	10x96T	$7932.50		Add to Cart

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