Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of CIC and the recovery rates were calculated by comparing the measured value to the expected amount of CIC in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
97
EDTA plasma(n=5)
88-105
95
heparin plasma(n=5)
87-102
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CIC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
85-105%
90-104%
EDTA plasma(n=5)
82-100%
83-95%
85-95%
heparin plasma(n=5)
80-95%
80-89%
85-95%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
CIC ELISA Kit allows for the in vitro quantitative determination of CIC concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse CIC (Circulating Immune Complex ) ELISA Kit