Mouse CHL1/L1CAM-2 ELISA Kit PicoKine®

Mfr.No.

EK1577

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10000
O.D.	0.003	0.084	0.144	0.268	0.522	0.945	1.719	2.493

Description

Mouse CHL1/L1CAM-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Chl1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neural cell adhesion molecule L1-like protein;Cell adhesion molecule with homology to L1CAM;Chl1-like protein;Close homolog of L1;Processed neural cell adhesion molecule L1-like protein;Chl1;Call;

Molecular Weight

135074 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E26-W1083

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CHL1

Subcellular Localization

Cell membrane; Single-pass type I membrane protein. Soluble forms produced by cleavage/shedding also exist.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	218	1822	4674	207	1753	4970
Standard deviation	15.04	116.6	198.2%	17.59	143.74	238.56
CV (%)	6.9%	6.4%	4.2%	8.5%	8.2%	4.8%

Protein Function

Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons as well as in regulation of both the number of inteeurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane. .

Background

Neural cell adhesion molecule L1-like protein also known as close homolog of L1 (CHL1) is a protein that in humans is encoded by the CHL1 gene. This protein is a member of the L1 gene family of neural cell adhesion molecules. By FISH, the CHL1 gene was mapped to chromosome 3p26.1. The protein however has dynamic localisation, meaning that it has not only multiple roles in the cell, but also various locations. This protein may also play a role in the growth of certain cancers.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	218	223	245	253	234	14.63	6.2%
Sample 2	1822	1615	1911	1772	1780	107.48	6%
Sample 3	4674	4640	4837	4877	4757	101.7	2.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12661
Gene Name	Chl1
UniProt ID	P70232
Protein Name	Neural cell adhesion molecule L1-like protein
Tissue Specificity	Expressed in the brain, in the cerebellum and in the spinal cord. Detected in the retina and the optic nerve. Expressed in neurons and glial cells in the central nervous system and by Schwann cells in the peripheral nervous system. .

Applications	ELISA
Research Area	Actin Binding Proteins,Actin, etc.,Cell Adhesion Proteins,Cytoskeleton,Cytoskeleton/ECM,ECM Proteins,Membrane Proteins,Microfilaments,Neurogenesis,Neurology Process,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001155	EK1577	96 wells/kit	$750		Add to Cart

Mouse CHL1/L1CAM-2 ELISA Kit PicoKine®

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