Mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) ELISA kit

Mouse chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1) ELISA kit

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Mfr.No.

CSB-E17286m

Alternative Names

chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1), FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1, GRO1 oncogene (melanoma growth stimulating activity, alpha), GRO1 oncogene (melanoma growth-stimulating activity), MGSA alpha, chemokine (C-X-C motif) ligan

Reactivity

Mouse

Detection Range

7.8 pg/mL-500 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat ER spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	94-109
EDTA plasma (n=4)	92	88-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ER in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	86-91
1:2	Average %	89
	Range %	81-95
1:4	Average %	98
	Range %	93-104
1:8	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.915	2.731	2.823	2.700
1000	2.221	2.177	2.199	2.076
500	1.374	1.455	1.415	1.292
250	0.786	0.810	0.798	0.675
125	0.488	0.496	0.492	0.369
62.5	0.324	0.340	0.332	0.209
31.25	0.193	0.187	0.190	0.067
0	0.119	0.126	0.123

Species

Mus musculus

Sensitivity

1.95 pg/mL

Limit Of Detection

450 nm

UniProt ID	P12850
Antigen	chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) (CXCL1)

Applications

ELISA

Research Area

CXCL1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505371	CSB-E17286m	96T	$790	Add to Cart
BHE10505371	CSB-E17286m	5x96T	$3792	Add to Cart
BHE10505371	CSB-E17286m	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.