Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Chat and the recovery rates were calculated by comparing the measured value to the expected amount of Chat in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-101
93
EDTA plasma(n=5)
93-104
97
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chat and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Chat ELISA Kit allows for the in vitro quantitative determination of Chat concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Mouse Chat (Choline O-acetyltransferase) ELISA Kit