Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®

Mfr.No.

EK1420

Sensitivity

<20 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Mouse

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.131	0.211	0.242	0.355	0.535	0.889	1.466	2.017

Description

Mouse Adipsin / CFD / Factor D ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cfd in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Complement factor D; 28 kDa adipocyte protein; Adipsin; C3 convertase activator; Properdin factor D; Cfd; And; Df

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I26-S259

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CFD

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1435	4423	19418	1328	4783	18803
Standard deviation	107.62	309.61	1378.67	111.55	411.33	1541.84
CV (%)	7.5%	7%	7.1%	8.4%	8.6%	8.2%

Protein Function

Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alteate pathway. Its function is homologous to that of C1s in the classical pathway.

Background

Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alteative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. Whats more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1435	1482	1554	1340	1452	77.67	5.3%
Sample 2	4423	4063	4082	3952	4130	176.29	4.2%
Sample 3	19418	20714	18137	20289	19639	985.26	5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	11537
Gene Name	Cfd
UniProt ID	P03953

Applications	ELISA
Research Area	Mesenchymal Stem Cells,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001303	EK1420	96 wells/kit	$499		Add to Cart

Mouse CFD/Adipsin/Complement Factor D ELISA Kit PicoKine®

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