Matrices listed below were spiked with certain level of CD9 and the recovery rates were calculated by comparing the measured value to the expected amount of CD9 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-105
99
EDTA plasma(n=5)
92-102
96
heparin plasma(n=5)
92-99
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
87-104%
87-104%
EDTA plasma(n=5)
90-100%
89-100%
94-100%
heparin plasma(n=5)
94-98%
94-100%
95-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P40240
Applications
ELISA
Applications Notes
CD9 ELISA Kit allows for the in vitro quantitative determination of CD9 concentrations in serum, plasma, tissue homogenates and other biological fluids.