Mouse CD70 antigen(CD70) ELISA kit

Mouse CD70 antigen(CD70) ELISA kit

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Mfr.No.

CSB-EL004954MO

Sensitivity

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Detection Range

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Reactivity

Mouse

Assay Duration

1-5h

Alternative Names

CD70 molecule, CD27L, CD27LG, TNFSF7, CD27 ligand, CD70 antigen, Ki-24 antigen, surface antigen CD70, tumor necrosis factor (ligand) superfamily, member 7, tumor necrosis factor ligand superfamily, member 7

Recovery

The recovery of rat HDL-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	107	101-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat HDL-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	87
	Range %	83-89
1:400	Average %	99
	Range %	95-102
1:800	Average %	90
	Range %	85-93
1:1600	Average %	99
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
6000	2.612	2.554	2.583	2.483
3000	2.122	2.158	2.140	2.040
1500	1.449	1.453	1.451	1.351
750	0.845	0.831	0.838	0.738
375	0.420	0.434	0.427	0.327
187.5	0.272	0.278	0.275	0.175
93.75	0.176	0.169	0.173	0.073
0	0.101	0.099	0.100

Species

Mus musculus

Limit Of Detection

450 nm

Precision

UniProt ID	O55237

Applications	ELISA
Research Area	CD70

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10505349	CSB-EL004954MO	96T	$695	Add to Cart
BHE10505349	CSB-EL004954MO	5x96T	$3336	Add to Cart
BHE10505349	CSB-EL004954MO	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.