Mouse CD48/Slamf2 ELISA Kit PicoKine®

Mfr.No.

EK1314

Sensitivity

<10 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0	0.026	0.063	0.14	0.379	0.917	1.707	2.073

Description

Mouse CD48/Slamf2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Cd48 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

CD48 antigen;BCM1 surface antigen;BLAST-1;HM48-1;MRC OX-45 surface antigen;SLAM family member 2;SLAMF2;Signaling lymphocytic activation molecule 2;sgp-60;CD48;Cd48;Bcm-1;

Molecular Weight

27383 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: F23-S217

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CD48

Subcellular Localization

Cell membrane; Lipid-anchor, GPI-anchor.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	91	816	1332	95	736	1273
Standard deviation	4	34.27	87.91	5.03	41.21	98.02
CV (%)	4.4%	4.2%	6.6%	5.3%	5.6%	7.7%

Protein Function

Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Background

CD48 antigen (Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily (IgSF) which includes SLAM (signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	91	79	95	86	87	5.97	6.8%
Sample 2	816	795	773	755	784	22.93	2.9%
Sample 3	1332	1482	1540	1536	1472	84.28	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	12506
Gene Name	CD48
UniProt ID	P18181
Protein Name	CD48 antigen
Sequence	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Applications	ELISA
Research Area	Cell Type Markers,Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000903	EK1314	96 wells/kit	$750		Add to Cart

Mouse CD48/Slamf2 ELISA Kit PicoKine®

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