Mouse CCL28 ELISA Kit PicoKine®

Mfr.No.

EK0691

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Mouse

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.01	0.092	0.152	0.29	0.512	0.897	1.375	2.040

Description

Mouse CCL28 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ccl28 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 28;Small-inducible cytokine A28;Ccl28;Scya28;

Molecular Weight

14570 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I23-R130

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CCL28

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	79	209	835	80	199	814
Standard deviation	3.39	11.07	61.79	4.88	12.93	66.74
CV (%)	4.3%	5.3%	7.4%	6.1%	6.5%	8.2%

Protein Function

Chemotactic for resting CD4, CD8 T-cells and eosinophils (By similarity). Binds to CCR10 and induces calcium mobilization in a dose-dependent manner. .

Background

CCL28, also known as mucosae-associated epithelial chemokine (MEC), CCK1 and SCYA28, is a chemokine. It is mapped to 5p12. The tumor hypoxia promotes the recruitment of regulatory T (Treg) cells through induction of expression of the chemokine CCL28, which in tu promotes tumor tolerance and angiogenesis. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	79	84	92	78	83	5.53	6.6%
Sample 2	209	201	187	203	200	8.06	4%
Sample 3	835	758	831	907	832	52.69	6.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	56838
Gene Name	CCL28
UniProt ID	Q9JIL2
Protein Name	C-C motif chemokine 28
Tissue Specificity	Mainly expressed in testis, epithelial cells of normal colon, kidney, Peyer patches, lymph nodes. Also found in lower levels in brain, spleen and lung.

Applications	ELISA
Research Area	Chemokines,Cytokines,Immunology,Innate Immunity,Metabolism,Obesity

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000568	EK0691	96 wells/kit	$499		Add to Cart

Mouse CCL28 ELISA Kit PicoKine®

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